بررسی تاثیر سلول‌های اپی‌تلیالی پرده آمنیونی انسانی بر روند تولید وتکامل سلول‌های دندریتیک از منشاء مونوسیت‌های خون محیطی

Abstract Background: Various studies indicated that epithelial cells derived from the amniotic membranes of human term placenta have inhibitory effect on components of the innate or acquired immune system. On the other hand, during pregnancy one of the most inhibitory mechanisms of fetal survival maybe though to close contact between AECs and maternal myeloid derived (DCs) that leads to generate immunological tolerance avoid from tissue graft rejection by maternal immune defense. Materials and methods: In the presence study we look out for generating tollerogenic DCs from human monocytes derived iDCs by co-culturing with human term amniotic isolated epithelial cells to demonstrate inhibitory effect of AECs on phenotyping changes and maturation of cultured DCs. This change included: expression of immature (CD14, CD1a) or mature (CD80, CD86, CD83) CD markers and MHC I, II by flow cytometer technique. The cultivation system improved by addition of LPS, IL4&GM-CSFgrowth factors and insert chamber contact manner during 7 days culture.Cytokine research was done by ELISA method (IL12,IL10). Results: The surface expression of CD markers CD14, CD1a, CD80, CD86, CD83, HLA-DR and CD40) and secreted cytokines (IL10 and IL12) of cultural MDDCs during maturation of mature and immature DCs between two groups: test and controls (in the absent of AECs) no significant differences were observed. Conclusion: Any effects have been concluded from maturation and differentiation of MDDCs by co-culture with hAECs. Growing on future study by various changes in culture system our suggestion is implicate. Keywords: Human amniotic epithelial cells, Tollerogenic dendritic Cells, Immunosuppresion, Monocyte derived dendritic cells (MDDCs).